R&D Centre for Excellence

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R&D has professionals with proven expertise in Phytochemistry, Analytical chemistry, Molecular biology, Microbiology and Pharmacology

Research & Publications

A double blind, placebo-controlled randomized comparative study on the efficacy of phytosterol-enriched and conventional saw palmetto oil in mitigating benign prostate hyperplasia and androgen deficiency

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Kinetics and computational analysis of cholinesterase inhibition by REVERC3, a bisdemethoxycurcumin-rich Curcuma longa extract: Relevance to the treatment of Alzheimer’s disease
By Sudeep HV, Amritha Raj,
Gouthamchandra K, Chandrappa S
and Shyamprasad K

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United States Patent - SUN-CA for Alzheimer's Disease

Chlorogenic Acid Composition and Method for Its use in the treatment of Alzheimer's Disease
Patent No. US10,588,885

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Molecular docking analysis of Withaferin A from Withania somnifera with the Glucose regulated protein 78 (GRP78) receptor and the SARS-CoV-2 main protease

Key words: COVID-19, GRP78, Mpro, Withaferin A

Patent No. US10,588,885

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Sub-acute and acute toxicity of Ferula asafoetida and Silybum marianum

Ramanaiah I, Sudeep HV, David D, Shyamprasad K. Sub-acute and acute toxicity of Ferula asafoetida and Silybum marianum formulation and effect of the formulation on delaying gastric emptying. BMC Complem Altern M 2019; 19: 159. http://doi.org/10.1186/s12906-019-2576-7.

an alkaloid from Coffee arabica by HPLC method

Naveen P, Lingaraju HB, Deepak M, Medhini B, Shyam Prasad K. Method development and validation for the determination of caffeine, an alkaloid from Coffee arabica by HPLC method. Phcog Res 2018; 10: 88-91.

determination of costunolide from Cinnamomum tamala

Naveen P, Lingaraju HB, Priya KS, Shyam Prasad K. Method development and validation for the determination of costunolide from Cinnamomum tamala by HPLC method. Phcog Res 2018; 10: 422-425.

anticancer effects against cultured human colon cancer

Gouthamchandra K, Sudeep HV, Venkatesh BJ, Shyamprasad K. Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells. Food Science and Human Wellness 2017; 6(3): 147-153. https://doi.org/10.1016/j.fshw.2017.06.001

Hydroalcoholic extract from Lepidium meyenii

Bramara BVB, Vasavi HS, Sudeep HV, Shyamprasad K. Hydroalcoholic extract from Lepidium meyenii (Black maca) root exerts wound healing activity in Streptozotocin-induced diabetic rats. Wound Medicine 2017; 19: 75-81. https://doi.org/10.1016/j.wndm.2017.10.003

Bioavailability-enhanced ResveramaxTM

Vasavi HS, Sudeep HV, Lingaraju HB, Shyamprasad K. Bioavailability-enhanced ResveramaxTM modulates quorum sensing and inhibits biofilm formation in Pseudomonas aeruginosa PAO1. Microb Pathog 2017; 104: 64-71. doi: 10.1016/j.micpath.2017.01.015.

standardized Withania somnifera

Sudeep HV, Gouthamchandra K, Venkatesh BJ, Shyamprasad K. Viwithan, a standardized Withania somnifera root extract induces apoptosis in murine melanoma cells. Phcog Mag 2017; 13: S801-6. doi: 10.4103/pm.pm_121_17

Validation for the Determination of Caffeine

Naveen P, Lingaraju HB, Deepak M, Medhini B, Shyam Prasad K. Method Development and Validation for the Determination of Caffeine, an alkaloid from Coffee arabica by HPLC method. Phcog Res 2017.

Biomechanism of chlorogenic acid

Sudeep HV, Venkatakrishna K, Dipak Patel, Shyamprasad K. Biomechanism of chlorogenic acid complex mediated plasma free fatty acid metabolism in rat liver. BMC Complem Altern M 2016; 16: 274. doi: 10.1186/s12906-016-1258-y.

MICROBIAL PATHOGENESIS

Bioavailability-enhanced ResveramaxTM modulates quorum sensing and inhibits biofilm formation in Pseudomonas aeruginosa

INTERNATIONAL JOURNAL OF CURRENT RESEARCH

The polyphenols in Pomegranate play important role in exhibiting anti-ageing effects

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JOURNAL OF CHEMICAL & PHARMACEUTICAL RESEARCH

Computational studies on the anti-obesity effect of polyphenols from Pomegranate leaf.

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JOURNAL OF PHARMACY RESEARCH

Effect of chlorogenic acid content in roasted coffee on in vitro antioxidant efficiency

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E-JOURNAL OF CHEMISTRY

Evaluation of antioxidant activity of medicinal plant extracts produced for commercial purpose

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INTERNATIONAL JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES

Antimicrobial efficacy of some natural cosmeceuticals, neutraceuticals and medicinal plant extracts and ultrastructural alterations in food borne pathogens

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Effect of Coleus forskohlii extract on the induction of hepatic cytochrome

Reethi B, Sudeep HV, Lingaraju HB, Shyamprasad K. Effect of Coleus forskohlii extract on the induction of hepatic cytochrome P450 isoenzyme CYP 1A in rats. World Journal of Pharmacy and Pharmaceutical Sciences 2015; 4(1): 1185-1192.

Rapid development and validation of improved reversed-phase

Naveen P, Lingaraju HB, Shyamprasad K. Rapid development and validation of improved reversed-phase high-performance liquid chromatography method for the quantification of mangiferin, a polyphenol xanthone glycoside in Mangifera indica. Phcog Res 2017; 9(2): 215-219. doi: 10.4103/0974-8490.204652

Simultaneous determination of rutin, isoquercetin

Naveen P, Lingaraju HB, Anitha, Shyamprasad K. Simultaneous determination of rutin, isoquercetin, and quercetin flavonoids in Nelumbo nucifera by high-performance liquid chromatography method. Int J Pharma Investig 2017; 7: 94-100.

Viwithan, a standardized Withania somnifera root extract

Sudeep HV, Gouthamchandra K, Venkatesh BJ, Shyamprasad K. Viwithan, a standardized Withania somnifera root extract induces apoptosis in murine melanoma cells. Phcog Mag 2017; 13: S801-6. doi: 10.4103/pm.pm_121_17

Bioavailability-enhanced ResveramaxTM modulates quorum sensing

Vasavi HS, Sudeep HV, Lingaraju HB, Shyamprasad K. Bioavailability-enhanced ResveramaxTM modulates quorum sensing and inhibits biofilm formation in Pseudomonas aeruginosa PAO1. Microb Pathog 2017; 104: 64-71. doi: 10.1016/j.micpath.2017.01.015.

Hydroalcoholic extract from Lepidium meyenii (Black maca)

Bramara BVB, Vasavi HS, Sudeep HV, Shyamprasad K. Hydroalcoholic extract from Lepidium meyenii (Black maca) root exerts wound healing activity in Streptozotocin-induced diabetic rats. Wound Medicine 2017; 19: 75-81. https://doi.org/10.1016/j.wndm.2017.10.003

Chlorogenic acid complex (CGA7)

Gouthamchandra K, Sudeep HV, Venkatesh BJ, Shyamprasad K. Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells. Food Science and Human Wellness 2017; 6(3): 147-153. https://doi.org/10.1016/j.fshw.2017.06.001

Method development and validation for the determination

Naveen P, Lingaraju HB, Priya KS, Shyam Prasad K. Method development and validation for the determination of costunolide from Cinnamomum tamala by HPLC method. Phcog Res 2018; 10: 422-425.

Sub-acute and acute toxicity of Ferula asafoetida and Silybum marianum

Ramanaiah I, Sudeep HV, David D, Shyamprasad K. Sub-acute and acute toxicity of Ferula asafoetida and Silybum marianum formulation and effect of the formulation on delaying gastric emptying. BMC Complem Altern M 2019; 19: 159. http://doi.org/10.1186/s12906-019-2576-7.

SUNCA standardized extract from Helianthus annus

Gouthamchandra K, Sudeep HV, Amritharaj, Lingaraju HB, Shyamprasad K. SUNCA standardized extract from Helianthus annus L exerts enhanced digestive enzyme activity and subsides obesity through inhibition of C/EBP-α and PPAR-γ expression both in vitro and in vivo model. Pharmacogn. Mag 2019; 15: S538-44.

phytosterol-enriched saw palmetto supercritical CO2 extract

Sudeep HV, Venkatakrishna K, Amrutharaj B, Anitha, Shyamprasad K. A phytosterol-enriched saw palmetto supercritical CO2 extract ameliorates testosterone-induced benign prostatic hyperplasia by regulating the inflammatory and apoptotic proteins in a rat model. BMC Complem Altern M 2019; 19: 270. http://doi.org/10.1186/s12906-019-2697-z.

Development and validation of veratric acid in Tabebuia avellanedae

Naveen P, Lingaraju HB, Chandrappa S, Anitha S, Shyamprasad K. Development and validation of veratric acid in Tabebuia avellanedae using liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry-multiple reaction monitoring-based assay coupled with 1,1-diphenyl-2-picrylhydrazyl method. Phcog Res 2020; 12: 107-11. Doi: 10.4103/pr.pr_77_19

Molecular docking analysis of Withaferin A from Withania somnifera

Sudeep HV, Gouthamchandra K, Shyamprasad K. Molecular docking analysis of Withaferin A from Withania somnifera with the glucose regulated protein 78 (GRP78) receptor and the SARS-CoV2 main protease. Bioinformation 2020; 16(5): 411-417. Doi: 10.6026/97320630016411.

A double blind, placebo-controlled randomized comparative study on the efficacy of phytosterol-enriched

Sudeep HV, Jestin V Thomas, Shyamprasad K. A double blind, placebo-controlled randomized comparative study on the efficacy of phytosterol-enriched and conventional saw palmetto oil in mitigating benign prostate hyperplasia and androgen deficiency. BMC Urol 2020; 20: 86. http://doi.org/10.1186/s12894-020-00648-9

Kinetics and computational analysis of cholinesterase inhibition by REVERC3

Sudeep HV, Amritha Raj, Gouthamchandra K, Chandrappa S, Shyamprasad K. Kinetics and computational analysis of cholinesterase inhibition by REVERC3, a bisdemethoxycurcumin-rich Curcuma longa extract: Relevance to the treatment of Alzheimer’s disease. SAGE Open Med 2020; 8: 1-9.

Potential benefits of oral administration of Amorphophallus konjac glycosylceramides

Sudeep HV, Naveen P, Shyamprasad K. Potential benefits of oral administration of Amorphophallus konjac glycosylceramides on skin health – a randomized clinical study. BMC Complem Med Ther 2020; 20: 26. http://doi.org/10.1186/s12906-019-2721-3

A standardized bisacurone-rich turmeric rhizome extract for the prevention and treatment of hangover and alcohol-induced

Sudeep HV, Venkatakrishna K, Sudeep K, Vasavi HS, Amritharaj, Chandrappa S, Shyamprasad K. Turcuron: A standardized bisacurone-rich turmeric rhizome extract for the prevention and treatment of hangover and alcohol-induced liver injury in rats. Phcog Mag 2020; 16: S263-71.

Viwithan: a standardized Ashwagandha extract ameliorates ovalbumin-induced airway-inflammation

Vasavi HS, Sudeep HV, Ramanaiah I, Shyamprasad K. Viwithan: a standardized Ashwagandha extract ameliorates ovalbumin-induced airway-inflammation and oxidative stress in mouse model. 2021; 14(3): 89-93

Isolation, method development, and validation of diterpenes in coffee oil

Naveen P, Lingaraju HB, Chandrappa S, Deepak M, Rashmi R, Shyamprasad K. Isolation, method development, and validation of diterpenes in coffee oil: identification by multiple reaction monitoring approach with atmospheric pressure chemical ionization mode. Phcog Res 2021; 13: 42-8. Doi: 10.4103/pr.pr_66_20

Bioactive compound bisacurone in the turmeric extract (turcuron) prevents non-alcoholic fatty liver disease

Gouthamchandra K, Sudeep HV, Amritha Raj, Ramanaiah I, Chandrappa S, Shyamprasad K. Bioactive compound bisacurone in the turmeric extract (turcuron) prevents non-alcoholic fatty liver disease by reduction of lipogenesis. IJPER 2021; 55(1s): s48-s55. doi:10.5530/ijper.55.1s.36

In vitro gastrointestinal digestion of a bisdemethoxycurcumin-rich Curcuma longa extract

Sudeep HV, Gouthamchandra K, Chandrappa S, Naveen P, Reethi B, Venkatakrishna K, Shyamprasad K. In vitro gastrointestinal digestion of a bisdemethoxycurcumin-rich Curcuma longa extract and its oral bioavailability in rats. Bull Natl Res Cent 2021; 45: 84. https://doi.org/10.1186/s42269-021-00544-8

Efficacy of a standardized turmeric extract comprised of 70% bisdemethoxy-curcumin (REVERC3)

Gouthamchandra K, Sudeep HV, Chandrappa S, Amrith Raj, Naveen P, Shyamprasad K. Efficacy of a standardized turmeric extract comprised of 70% bisdemethoxy-curcumin (REVERC3) against LPS-induced inflammation in Raw 264.7 cells and carrageenan-induced paw edema. J Inflamm Res. 2021; 14: 859-868.

Acute and sub-chronic toxicity evaluation of a standardized green coffee bean extract (CGA-7TM)

Venkatakrishna K, Sudeep HV, Shyamprasad K. Acute and sub-chronic toxicity evaluation of a standardized green coffee bean extract (CGA-7TM) in Wistar albino rats. SAGE Open Med 2021; 9: 1-12. Doi: 10.1177/2050312120984885

Supplementation of green coffee bean extract in healthy overweight subjects increases lean mass/fat mass ratio

Sudeep HV, Shyamprasad K. Supplementation of green coffee bean extract in healthy overweight subjects increases lean mass/fat mass ratio: A randomized, double-blind clinical study. SAGE Open Med 2021; 9: 1-10. Doi: 10.1177/20503121211002590

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